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Borszczagowski line (B), destined to field cultivation and its line derived in the course of recurrent regenerations in in vitro culture (BRR) were used in the study. Cucumber plants were grown at 30 × 120 cm, thirty plants on each plot. During vegetation, length measurements of shoots were made and female and male flowers were counted. Fruits were collected several times. Total and marketable yields and fruits quality were estimated. The results of three-year study indicate that cucumber plants regenerated in vitro show variability on a molecular level comparing to Borszczagowski line. Multiple regeneration in vitro significantly affected both permanent variability of developmental and field parameters and physical and chemical traits of line (BRR) cucumber fruits.
Key words: somaclonal variability, yield, physical traits, chemical composition