Department of Vegetable and Medicinal Plants

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Basic Chemical Analysis Lab

Research
This lab is used for the qualitative and quantitative analysis of the chemical composition of plant material (fresh, frozen and dried vegetables and herbs), and extracts obtained by different methods of extraction.
   The influence of the cultivation, harvesting and storage conditions on the content of basic chemicals in these products is evaluated. Second purpose is determination of the relationship between the development of plants growing wild and in cultivation conditions and their chemical composition and content of certain biologically active compounds with special emphasis on the diversification of genetic material.
   This lab is also used for the teaching purpose of the courses Vegetables quality assessment, Instrumental analysis of plant raw materials, Instrumental analysis, Methods of drying and preservation of vegetable and herb raw materials and Sustainable horticulture.
Equipment
  • Flame Photometer Model 410

    Determination of Ca and K

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  • FOSS FIAstar 5000 Spektrofotometer

    Determination of N-N03, N-N02, N-NH4 & Mg

  • Shimadzu 1800 UV-Vis Spectrophotometer, resolution 1 nm, double-beam optics, wavelength range of 190 to 1100 nm, temperature controller (7-60° Celsius)

    Quantitative analysis: protein, flawonoids, phenolic acids, cumarins, sterols, tannin etc.

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  • Shimadzu 1700 PharmaSpec UV-Vis Spectrophotometer, double-beam optics, wavelength range of 190 to 1100 nm, temperature controller (7-60° Celsius)

    Quantitative analysis: protein, flawonoids, phenolic acids, cumarins, sterols, tannin etc.

  • Cecil 3021 Super Aurius Series UV-Vis Spectrophotometer, wavelength range of 190 to 1000 nm

    Quantitative analysis: flawonoids, phenolic acids, cumarins, sterols, tannin etc.

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  • Refractometers

    Determination of sugar extract

  • pH and Conductivity Meters

    Determination of pH and soil conductivity

  • Refrigerated Eppendorf centrifuge, digestoria, upright -80, -20, 4°C freezers, dryers, microwaves, shakers, balances

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