Department of Vegetable and Medicinal Plants

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Instrumental Analysis Lab

Research
Quantitative and qualitative analysis of key biologically active compounds in raw materials and extracts obtained from experiments is kept in this lab. The investigation concerning the various groups of compounds, in particular essential oils, flavonoids, poliphenolic acids, trans-cinnamic alcohol derivatives, catechin, coumarin, sterols etc.
   Determined is the influence of the cultivation, harvesting and storage conditions on the content of these substances in fresh, frozen and dried vegetables and herbs as well as plant extracts obtained by various methods. The relationship between the development of plants growing wild in their natural habitat and in cultivation conditions and the content of these substances with special emphasis on the genetic material diversification is also determined.
   This lab is also used for the teaching purpose of the courses Instrumental analysis of plant raw materials, Instrumental analysis, Vegetables quality assessment, Methods of drying and preservation of vegetable and herb raw materials and Sustainable horticulture.
Equipment
  • Shimadzu LC-20A Prominence UFLC HPLC with diode array detector (DAD), fluorescence Detector (FLD) and evaporative light scattering detector (ELSD), post-column derivatization kit

    Separation & quantification of non-volatile organic compounds: carotenoids, flavonoids, poliphenolic acids, trans-cinnamic alcohol derivatives, catechin, coumarin, organic acids, sugars, sterols etc.

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  • Shimadzu LC-6 HPLC with diode array detector (DAD)

    Separation & quantification of non-volatile organic compounds: flavonoids, poliphenolic acids, trans-cinnamic alcohol derivatives, catechin, coumarin, sterols etc.

  • Shimadzu LC-8 preparative HPLC with UV-Vis detector

    Separation of non-volatile organic compounds: flavonoids, poliphenolic acids, trans-cinnamic alcohol derivatives, catechin, coumarin, sterols etc.

  • Hewlett-Packard 6890 GC with flame ionization detector (FID)

    Quantification of volatile organic compounds: volatile oils, fatty acids

  • Solid phase microextraction (SPME)
  • Camag TLC equipment: semi-automatic sample application system Linomat 5, Automatic Developing Chamber ADC 2, wetrical & horizontal developing chambers, derivatization set, documentation system Reprostar 3 with winCATS & VideoScan software

    entry evaluation of raw material, extracts and volatiles oils

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  • Water purification system Cobrabid Aqua

    WCA R03 DP ECO

  • Anatyrical scale Ohaus

    Pionieer PA214CM⁄1 e=0,001g d=0,0001 g

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