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Instrumental Analysis Lab
Research
Quantitative and qualitative analysis of key biologically active compounds in raw materials and extracts obtained from experiments is kept in this lab. The investigation concerning the various groups of compounds, in particular essential oils, flavonoids, poliphenolic acids, trans-cinnamic alcohol derivatives, catechin, coumarin, sterols etc.
Determined is the influence of the cultivation, harvesting and storage conditions on the content of these substances in fresh, frozen and dried vegetables and herbs as well as plant extracts obtained by various methods. The relationship between the development of plants growing wild in their natural habitat and in cultivation conditions and the content of these substances with special emphasis on the genetic material diversification is also determined.
This lab is also used for the teaching purpose of the courses Instrumental analysis of plant raw materials
, Instrumental analysis
, Vegetables quality assessment
, Methods of drying and preservation of vegetable and herb raw materials
and Sustainable horticulture
.
Equipment
Shimadzu LC-20A Prominence UFLC HPLC with diode array detector (DAD), fluorescence Detector (FLD) and evaporative light scattering detector (ELSD), post-column derivatization kit
Separation & quantification of non-volatile organic compounds: carotenoids, flavonoids, poliphenolic acids, trans-cinnamic alcohol derivatives, catechin, coumarin, organic acids, sugars, sterols etc.
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Shimadzu LC-6 HPLC with diode array detector (DAD)
Separation & quantification of non-volatile organic compounds: flavonoids, poliphenolic acids, trans-cinnamic alcohol derivatives, catechin, coumarin, sterols etc.
Shimadzu LC-8 preparative HPLC with UV-Vis detector
Separation of non-volatile organic compounds: flavonoids, poliphenolic acids, trans-cinnamic alcohol derivatives, catechin, coumarin, sterols etc.
Hewlett-Packard 6890 GC with flame ionization detector (FID)
Quantification of volatile organic compounds: volatile oils, fatty acids
Solid phase microextraction (SPME)
Camag TLC equipment: semi-automatic sample application system Linomat 5, Automatic Developing Chamber ADC 2, wetrical & horizontal developing chambers, derivatization set, documentation system Reprostar 3 with winCATS & VideoScan software
entry evaluation of raw material, extracts and volatiles oils
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Water purification system Cobrabid Aqua
WCA R03 DP ECO
Anatyrical scale Ohaus
Pionieer PA214CM⁄1 e=0,001g d=0,0001 g
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